Exosomes are small extracellular vesicles and present in almost all types of body fluids. They can be loaded with various materials such as proteins, RNAs and DNA, therefore they are crucial for cell-cell communication by transmitting signals to regulate diverse cellular functions in recipient cells present at particular sites. They can also exert a significant impact on the development of disease processes. Recently, exosomes are becoming a hot topic for research, especially in the fields of disease diagnosis, prognosis, therapy, and biomarker discovery. The isolation and purification of exosomes from biological fluids is a prerequisite step in exosome research. High purity of exosomes is still technically challenging to achieve which can affect the outcome.