Human Chondrocyte Cell Line are the only cells found in articular cartilage. Since there are no vascular, nervous and lymphatic systems in articular cartilage, chondrocytes can survive in an anaerobic environment and get nutrients from the synovial fluid through diffusion. The chondrocytes provide mechanical support as a key functional component and permit smooth pain-free articulation in cartilage. Protein and gene expression, metabolic activity, and surface markers are common features of chondrocytes, and differences can be observed along the depth of cartilage tissue. Chondrocytes exhibit distinctive features, such as having metabolic activity to maintain the turnover of extracellular matrix (ECM) by synthesizing glycoprotein, collagens, proteoglycans, and hyaluronan.